Dissertation research: Mitochondrial transfers: Nuclear copies of mitochondrial fragments or numt pseudogenes are fragments of the mitochondrial DNA that have transferred from the mitochondrial genome to the nuclear genome. They have been identified in a variety of animals and plants but it is not known how or why these fragments integrate into the nucleus. Once identified, numts can be used to compare rates of mitochondrial and nuclear evolution and numt insertion sites can be examined to assess whether numts preferentially integrate into certain regions of the genome. I discovered multiple numt transfers within the Microtus nuclear genome including one transfer that encompassed over 25% of its mitochondrial genome. I characterized one of the numts pseudogenes in 21 additional arvicoline species and estimated that the transfer to the nucleus occurred ~4 MYA, predating the origins of most arvicolines. After comparing numt integration within Microtus to that of Mus and Rattus, I found that numt integration appears to be more extensive in Microtus than in other rodents. |
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Publications Triant, D. A., Pace, R. M. & Stine M. 2004. Abundance, genetic diversity and conservation of Louisiana black bears (Ursus americanus luteolus) as detected through noninvasive sampling. Conservation Genetics 5:647-659. Triant, D. A. & DeWoody, J. A. 2006. Accelerated molecular evolution in Microtus (Rodentia) as assessed via complete mitochondrial genome sequences. Genetica, In press. Triant, D. A. & DeWoody, J. A. The occurrence, detection and avoidance of mitochondrial DNA translocations in mammalian systematics and phylogeography. Journal of Mammalogy, In press. Triant, D. A. & DeWoody, J. A. Molecular analyses of mitochondrial pseudogenes within the nuclear genome of arvicoline rodents. Genetica, In revision. Triant, D. A. & DeWoody, J. A. Extensive numt transfer in a rapidly evolving rodent has been mediated by independent insertion events and by duplications. Submitted. |