Purdue University Life Science Fluorescence Imaging Facility Purdue University Home page Purdue Agriculture Home pagePurdue Life Science Fluorescence Imaging Facility Purdue University College of Science Home page Equipment: Scheduling & Types

Equipment: Zeiss LSM 710, Nikon E800, Intavis InSitu ProVS

 

Confocal Laser Scanning Microscope

  • Zeiss LSM 710 138D Whistler
    This is the state-of-the-art upright confocal microscope. Spectral scanning, multi-tracking, multi-dimensional acquisition, Z-stack, time series, 2.5-D, 3-D, FRAP, FRET, Multi-time, ion concentration, ratiometric imaging.

    • Laser lines available:

    Laser                           Wavelengths                          
    Argon                          458, 488, 514     
    HeNe 543                    543                                                                 
    HeNe 594                    594                                                                             
    HeNe 633                    633                                                                             
    Diode 405-430           405 

     

  • Zeiss Imaging workstation 142 Whistler
    After you log off of the Zeiss LSM 710, your files are uploaded onto the server, and you can access them at the workstation through your OnePurdue account.
    The workstation allows you to do analyses of your data so that the confocal microscope is open for the next user.

    Data acquisition and processing:
    The microscopes are primarily for image acquisition. Images should be processed and analysed at the workstation or in your own lab whenever possible.
    Reference images are stored on the LSFIF computer so you can re-use your settings. After you have not been actively using the facility for 6 months, your data will be deleted without notification.

 

Epifluorescence Microscope

  • Nikon Eclipse 800 142 Whistler

    • This is a great tool for prescreening your samples. That way you do not have to spend extra time on the confocal confirming XFP fluorescence, etc.

    Filters available on the Nikon:

    DAPI (UV-2A) .............Ex 330 nm -380 nm, DM 400 nm, BA 420 nm (Em 420 nm long pass)

    FITC (B-2A) ................Ex 450 nm - 490 nm, DM 500 nm, BA 515 nm (Em 515 nm long pass)

    GFP ..............................Ex 450 nm - 490 nm, DM 495 nm, BA 500 nm - 530 nm (Em 5500 nm - 530 nm band pass)

    Rhodamine/Texas Red ....Ex 490 n , Em 650 nm

    This microscope is currently equipped with a Spot-2 CCD camera.

    Data acquisition and processing:
    The microscopes are primarily for image acquisition. Images should be processed and analysed in your own lab whenever possible.
    Data should be stored on your OnePurdue account. Youare responsible for safe backups of your data. After you have not been actively using the facility for 6 months, your data will be deleted without notification.

 

Robotics

  • Intavis InSitu ProVS S024 Whistler
    • The preparative robot can do dehydration series, hybridization incubations for in situs and immunolocalizations, and rehydration series. You can use whole tissues (in baskets) or sections (on slides). This labor saving device frees you from all those tedious steps so you can be doing more fun experiments. It is perfect for high through-put projects.


Bring Your Own Consumables


The facility is user driven, and so it’s BYOC: e.g. slides, cover slips, lens paper, immersion media, etc.
Below is ordering information for some less common items.